MWI(0) | 110 | kDa |
MWexpected | 69 | kDa |
VPorod | 186 | nm3 |
log I(s)
1.09×102
1.09×101
1.09×100
1.09×10-1
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s, nm-1
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Synchrotron SAXS data from solutions of Human Guanylate Binding Protein 1 with guanylyl imidodiphosphate (hGBP1 + GppNHp) in 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, 0.2 mM GppNHp, pH 7.9 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 22°C. 10 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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