| MWexperimental | 125 | kDa |
| MWexpected | 135 | kDa |
| VPorod | 216 | nm3 |
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log I(s)
3.59×10-2
3.59×10-3
3.59×10-4
3.59×10-5
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s, nm-1
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Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4.
Giménez-Mascarell P, Oyenarte I, González-Recio I, Fernández-Rodríguez C, Corral-Rodríguez MÁ, Campos-Zarraga I, Simón J, Kostantin E, Hardy S, Díaz Quintana A, Zubillaga Lizeaga M, Merino N, Diercks T, Blanco FJ, Díaz Moreno I, Martínez-Chantar ML, Tremblay ML, Müller D, Siliqi D, Martínez-Cruz LA, Int J Mol Sci 20(24) (2019) Europe PMCSASDEN8 – Intracellular region of the human Cyclin and CBS domain divalent metal cation transport mediator-4 in complex with Phosphatase of Regenerating Liver-1 and MgATP
CNNM4_BAT-cNMP-CtailProtein tyrosine phosphatase type IVA 1
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Fits and models
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Synchrotron SAXS data from solutions of the intracellular region of the human cyclin and CBS domain divalent metal cation transport mediator-4 in complex with phosphatase of regenerating Liver-1 and MgATP in HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH 7.4 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the B21 camera at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data obtained through the sample elution peak (collected as 620 successive 3 second frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.
SEC-SAXS was performed at 20°C using the following parameters: Column Shodex KW-403 ; Flow rate: 0.08 mL/min; Total acquisition time: 1860s; Sample injection concentration: 5.0 mg/mL; Injection volume: 45μL. |
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