cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity.

Liu H, Moura-Alves P, Pei G, Mollenkopf HJ, Hurwitz R, Wu X, Wang F, Liu S, Ma M, Fei Y, Zhu C, Koehler AB, Oberbeck-Mueller D, Hahnke K, Klemm M, Guhlich-Bornhof U, Ge B, Tuukkanen A, Kolbe M, Dorhoi A, Kaufmann SH, EMBO Rep (2019) Europe PMC

SASDEQ9 – Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP)

Cyclic GMP-AMP synthase
2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine

MW^experimental	98	kDa
MW^expected	124	kDa
V^Porod	127	nm³

log I(s) 2.54×10³ 2.54×10² 2.54×10¹ 2.54×10⁰

Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine Guinier plot

ln 2.54×10³ R_g: 3.9 nm 0 (3.9 nm)^-2 s²

(sR_g)²I(s)/I(0)

Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 4.1 nm 0 D_max: 14.1 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.8

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.001
1.109

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Cyclic GMP-AMP synthase (cGAS) with cyclic guanosine monophosphate–adenosine monophosphate (2'3'-cGAMP) Rg histogram

R_g, nm

Cyclic GMP-AMP synthase 2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine EOM/RANCH model

Synchrotron SAXS data from solutions of cyclic GMP-AMP synthase (cGAS) with 2'3'-cGAMP in 20 mM HEPES, pH 7.4 were measured using size-exclusion chromatography SAXS (SEC-SAXS) on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column: XXXX ; Flow rate: 0.5 mL/min; Total acquisition time: 3600 x 1 s frames; Sample injection concentration: 9 mg/mL; Injection volume: 75 μL. The data obtained through the sample elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Cyclic GMP-AMP synthase (cGAS)
Mol. type		Protein
Organism		Homo sapiens
Olig. state		Dimer
Mon. MW		61.4 kDa

UniProt		Q8N884 (1-522)
Sequence		FASTA

2'-O,5'-O-((adenosine-3'-O,5'-O-diyl)bisphosphinico)guanosine (2′3′-cGAMP)
Mol. type		Other
Olig. state		Dimer
Mon. MW		0.7 kDa
Chemical formula