Senescence-associated ubiquitin ligase 1 (SAUL1)

Haifa El Kilani, Al Kikhney.

SASDER3 – Senescence-associated E3 ubiquitin ligase 1, 1.8 mg/ml

Senescence-associated E3 ubiquitin ligase 1
MWexperimental 199 kDa
MWexpected 355 kDa
log I(s) 7.55×103 7.55×102 7.55×101 7.55×100
Senescence-associated E3 ubiquitin ligase 1 small angle scattering data  s, nm-1
ln I(s)
Senescence-associated E3 ubiquitin ligase 1 Guinier plot ln 7.56×103 Rg: 5.9 nm 0 (5.9 nm)-2 s2
(sRg)2I(s)/I(0)
Senescence-associated E3 ubiquitin ligase 1 Kratky plot 1.104 0 √3 sRg
p(r)
Senescence-associated E3 ubiquitin ligase 1 pair distance distribution function Rg: 6.2 nm 0 Dmax: 23 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Senescence-associated E3 ubiquitin ligase 1 SASREF MX model
Senescence-associated E3 ubiquitin ligase 1 SASREF MX model
Synchrotron SAXS data from solutions of Senescence-associated E3 ubiquitin ligase 1, 1.8 mg/ml in 50 mM Tris, 250 mM NaCl, pH 9 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.123987 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.80 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Monomer:tetramer fractions by volume: 60:40. The rigid bodies used for the SASREF MX tetramer model were predicted by I-TASSER.

Senescence-associated E3 ubiquitin ligase 1 (SAUL1)
Mol. type   Protein
Organism   Arabidopsis thaliana
Olig. state   Tetramer
Mon. MW   88.8 kDa
 
UniProt   Q9LM76
Sequence   FASTA