SANS
data from solutions of
ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc (SANS, 100% D2O)
in
30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH 7.5
were collected
using a MLZ detector
at a sample-detector distance of 5.5 m and
at a wavelength of λ = 5 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 3.00 mg/ml was measured
at 10°C.
One
7200 second frame was collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
ACA8 protein complex with Calmodulin encapsulated in a partially deuterated "stealth-nanodisc": (sND). Disc components (MSP1D1 scaffold protein - 75% deuterated, and POPC lipid - heads/tails 78% and 92% deuterated) are effectively matched out at 100% D2O. Thus the expected molecular weight should correspond to that of the ACA8:Calmodulin complex (~150 kDa). Data measured using a single instrument configuration: 5.5 m (sample-detector distances) with a fixed neutron wavelength (λ) of 5 Å.
s, nm-1
