| MWexperimental | 51 | kDa |
| MWexpected | 62 | kDa |
| VPorod | 90 | nm3 |
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log I(s)
2.08×102
2.08×101
2.08×100
2.08×10-1
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s, nm-1
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The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility.
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV, Nat Microbiol (2019) Europe PMCSASDEV7 – Stator protein complex FlaG/FlaF-I96Y
Stator protein FlaG soluble domainConserved flagellar protein FlaF-I96Y soluble domain
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Fits and models
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Synchrotron SAXS data from solutions of the stator protein complex FlaG/FlaF-I96Y in 25 mM citric acid/sodium citrate, 150mM NaCl, 3% glycerol, pH 3 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.5 and 3 mg/ml were measured at 10°C. 33 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Storage temperature = UNKNOWN |
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