| MWexperimental | 18 | kDa |
| MWexpected | 17 | kDa |
| VPorod | 25 | nm3 |
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log I(s)
1.18×103
1.18×102
1.18×101
1.18×100
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s, nm-1
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Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations.
Gijsbers A, Montagut DC, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N, Int J Mol Sci 19(12) (2018) Europe PMCSASDEW8 – Shwachman-Bodian-Diamond Syndrome protein (SDO1) with domains 2 and 3
Ribosome maturation protein SDO1|
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Fits and models
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Synchrotron SAXS data from solutions of Shwachman-Bodian-Diamond Syndrome protein (SDO1) with domains 2 and 3 in 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2, pH 8 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data obtained through the sample elution peak (collected as successive 1 second frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.
SEC-SAXS (using FPLC–Malvern TDA system ) was performed at 20°C using the following parameters: Column: Superdex 200 Increase 10/300GL ; Flow rate: 0.4 mL/min; Total acquisition time: 1850s; Sample injection concentration: 15 mg/mL; Injection volume: 50μL. |
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