Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative.

Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P, Biochemistry (2020) Europe PMC

SASDF22 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

somapacitan
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag
neonatal Fc receptor ectodomain alpha-chain
MWexperimental 130 kDa
MWexpected 131 kDa
VPorod 227 nm3
log I(s) 4.74×10-1 4.74×10-2 4.74×10-3 4.74×10-4
somapacitan FcRn binding optimised human serum albumin  V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain small angle scattering data  s, nm-1
ln I(s)
somapacitan FcRn binding optimised human serum albumin  V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Guinier plot ln 4.74×10-1 Rg: 4.2 nm 0 (4.2 nm)-2 s2
(sRg)2I(s)/I(0)
somapacitan FcRn binding optimised human serum albumin  V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Kratky plot 1.104 0 3 sRg
p(r)
somapacitan FcRn binding optimised human serum albumin  V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain pair distance distribution function Rg: 4.2 nm 0 Dmax: 14.7 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of the ternary complex of the FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan in 100 mM MES, 100 mM NaCl, pH 6.5 were collected on the I911-4 beam line at MAX IV (Lund, Sweden) using a Pilatus 1M detector at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.5 mg/ml was measured at 25°C. One 120 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

somapacitan
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   22.1 kDa
 
UniProt   P01241 (27-217)
Sequence   FASTA
 
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A (HSAopt)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   66.4 kDa
 
UniProt   P02768 (25-609)
Sequence   FASTA
 
neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag (FcRn ECD beta)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   12.6 kDa
 
UniProt   P61768 (21-119)
Sequence   FASTA
 
neonatal Fc receptor ectodomain alpha-chain (FcRn ECD alpha)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   30.4 kDa
 
UniProt   P55899 (24-297)
Sequence   FASTA