| MWexperimental | 230 | kDa |
| MWexpected | 145 | kDa |
| VPorod | 268 | nm3 |
|
log I(s)
1.53×102
1.53×101
1.53×100
1.53×10-1
|
s, nm-1
|
Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M, Nat Commun 11(1):2905 (2020) Europe PMCSASDF26 – DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 5.5 with 300 mM NaCl
80bp_DNA Forward80bp_DNA Reverse
DNA-binding protein HU-alpha
|
|
|
Fits and models
|
|
|
Synchrotron SAXS data from solutions of DNA-binding protein HU-alpha bound to 80 base-pair DNA in 10 mM Bis-Tris, 300 mM NaCl, pH 5.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). 300 successive 3 second frames were collected at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN. Concentration = UNKNOWN |
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||