Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.

Yadav DK, Zigáčková D, Zlobina M, Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ, Nucleic Acids Res (2019) Europe PMC

SASDF29 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1

Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4
ADP-ribosylation factor1 - short
MWexperimental 15 kDa
MWexpected 19 kDa
VPorod 21 nm3
log I(s) 4.40×10-1 4.40×10-2 4.40×10-3 4.40×10-4
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 ADP-ribosylation factor1 - short small angle scattering data  s, nm-1
ln I(s)
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 ADP-ribosylation factor1 - short Guinier plot ln 4.40×10-1 Rg: 1.7 nm 0 (1.7 nm)-2 s2
(sRg)2I(s)/I(0)
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 ADP-ribosylation factor1 - short Kratky plot 1.104 0 √3 sRg
p(r)
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 ADP-ribosylation factor1 - short pair distance distribution function Rg: 1.8 nm 0 Dmax: 6.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 ADP-ribosylation factor1 - short OTHER model
SAXS data from solutions of double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 in 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol, pH 6.8 were collected using a Rigaku BioSAXS-1000 instrument (CEITEC; Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.50 mg/ml was measured at 35°C. Six successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 (STAU1-RBD4)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   8.4 kDa
 
UniProt   O95793 (286-355)
Sequence   FASTA
 
ADP-ribosylation factor1 - short (ARF1 RNA - short)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   11.0 kDa
Sequence   FASTA