Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.

Yadav DK, Zigáčková D, Zlobina M, Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ, Nucleic Acids Res (2019) Europe PMC

SASDF39 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1

ADP-ribosylation factor1 - short
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4
MWexperimental 29 kDa
MWexpected 31 kDa
VPorod 39 nm3
log I(s) 1.90×10-1 1.90×10-2 1.90×10-3 1.90×10-4
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 small angle scattering data  s, nm-1
ln I(s)
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 Guinier plot ln 1.90×10-1 Rg: 2.1 nm 0 (2.1 nm)-2 s2
(sRg)2I(s)/I(0)
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 Kratky plot 1.104 0 √3 sRg
p(r)
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
ADP-ribosylation factor1 - short Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 OTHER model
SAXS data from solutions of double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 in 50 mM sodium phosphate buffer, 300 mM NaCl, 500 mM imidazole, pH 8 were collected using a Rigaku BioSAXS-1000 instrument (CEITEC; Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 35°C. Six successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ADP-ribosylation factor1 - short (ARF1 RNA - short)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   11.0 kDa
Sequence   FASTA
 
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 (STAU1-RBD34)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   19.7 kDa
 
UniProt   O95793 (183-355)
Sequence   FASTA