| MWI(0) | 125 | kDa |
| MWexpected | 143 | kDa |
| VPorod | 178 | nm3 |
|
log I(s)
4.98×103
4.98×102
4.98×101
4.98×100
|
s, nm-1
|
Molecular mechanism of leukocidin GH-integrin CD11b/CD18 recognition and species specificity.
Trstenjak N, Milić D, Graewert MA, Rouha H, Svergun D, Djinović-Carugo K, Nagy E, Badarau A, Proc Natl Acad Sci U S A (2019) Europe PMCSASDF45 – Leukocidin/Integrin alpha-M complex (LukGH-huCD11b-I) in the presence of a neutralizing antibody Fab fragment
Leukocidin GLeukocidin H
LukGH neutralizing antibody
Integrin alpha-M
|
|
|
Fits and models
|
|
|
Synchrotron SAXS data from solutions of the LukGH-huCD11b-I complex bound to a neutralizing antibody Fab fragment in 20 mM HEPES, 300 mM NaCl, 1 mM MgCl2, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.20 mg/ml was measured at 10°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||