| MWI(0) | 90 | kDa |
| MWexpected | 121 | kDa |
| VPorod | 152 | nm3 |
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log I(s)
5.74×103
5.74×102
5.74×101
5.74×100
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s, nm-1
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Molecular mechanism of leukocidin GH-integrin CD11b/CD18 recognition and species specificity.
Trstenjak N, Milić D, Graewert MA, Rouha H, Svergun D, Djinović-Carugo K, Nagy E, Badarau A, Proc Natl Acad Sci U S A (2019) Europe PMCSASDF55 – Leukocidin (LukGH) from Staphylococcus aureus in complex with a neutralizing antibody
Leukocidin GLeukocidin H
LukGH neutralizing antibody
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Fits and models
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Synchrotron SAXS data from solutions of LukGH from Staphylococcus aureus bound to a neutralizing antibody in 20 mM HEPES, 300 mM NaCl, 1 mM MgCl2, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 4.6 mg/ml were measured at 10°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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