Synchrotron SAXS data from solutions of RXR/RAR Heterodimer : N-CoRNID Complex (1:1) with RAR inverse agonist (BMS493) in 50mM Tris HCl, 150mM NaCl, 2mM TCEP, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.30 mg/ml was measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
BMS493 was added to the complex in a three molar excess before concentration (n.b., BMS493 = (E)-4-[2-[5,6-Dihydro- 5,5-dimethyl - 8- (2-phenylethynyl)naphthalen - 2- yl]ethen - 1- yl]benzoic acid, 4-[(1E)-2-[5,6 -Dihydro -5,5- dimethyl-8- (phenylethynyl)- 2-naphthalenyl]ethenyl]- benzoic acid).
s, nm-1
