Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless.

Ankush Jagtap PK, Müller M, Masiewicz P, von Bülow S, Hollmann NM, Chen PC, Simon B, Thomae AW, Becker PB, Hennig J, Nucleic Acids Res 47(8):4319-4333 (2019) Europe PMC

SASDF72 – roX2 RNA stem-loop 7 18mer-fragment

roX2 stem-loop 7, 18-mer fragment
MWexperimental 11 kDa
MWexpected 12 kDa
VPorod 14 nm3
log I(s) 2.63×101 2.63×100 2.63×10-1 2.63×10-2
roX2 stem-loop 7, 18-mer fragment small angle scattering data  s, nm-1
ln I(s)
roX2 stem-loop 7, 18-mer fragment Guinier plot ln 2.64×101 Rg: 1.8 nm 0 (1.8 nm)-2 s2
(sRg)2I(s)/I(0)
roX2 stem-loop 7, 18-mer fragment Kratky plot 1.104 0 √3 sRg
p(r)
roX2 stem-loop 7, 18-mer fragment pair distance distribution function Rg: 2.1 nm 0 Dmax: 8.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
roX2 stem-loop 7, 18-mer fragment DAMMIN model
Synchrotron SAXS data from solutions of the roX2 RNA stem-loop 7 18mer-fragment in 20 mM NaPO4, 200 mM NaCl, 1 mM DTT, pH 6.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

roX2 stem-loop 7, 18-mer fragment (SL7-18mer)
Mol. type   RNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   11.6 kDa
Sequence   FASTA