Synchrotron SAXS data from solutions of unposphorylated Ric-8A (amino acids 1-452) in 25mM HEPES, 150mM NaCl, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 25 °C using the following parameters: Column: Superdex 200 Increase 3.2/300 analytical SEC column; Flow rate: 0.05 mL/min; Sample injection concentration: 1.0 mg/mL; Injection volume: 100 μL. The data were collected through the SEC peak of the sample as a series of 5 x 1 second exposures. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.
CAUTION! The data used to calculate p(r) profile are different to primary SAXS data.
s, nm-1
