Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1.

Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI, J Biol Chem 294(37):13822-13829 (2019) Europe PMC

SASDFC4 – Condensin complex subunit 3-like protein, Ycg1, extrapolated to infinite dilution

Condensin complex subunit 3-like protein
MWI(0) 102 kDa
MWexpected 108 kDa
VPorod 236 nm3
log I(s) 7.92×103 7.92×102 7.92×101 7.92×100
Condensin complex subunit 3-like protein small angle scattering data  s, nm-1
ln I(s)
Condensin complex subunit 3-like protein Guinier plot ln 7.93×103 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Condensin complex subunit 3-like protein Kratky plot 1.104 0 √3 sRg
p(r)
Condensin complex subunit 3-like protein pair distance distribution function Rg: 4.6 nm 0 Dmax: 15.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Condensin complex subunit 3-like protein DAMMIF model
log I(s)
 s, nm-1
Condensin complex subunit 3-like protein SREFLEX model
Synchrotron SAXS data from solutions of Ycg1, extrapolated to infinite dilution, in 25 mM Tris 300 mM NaCl 1mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.3 and 0.5 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Condensin complex subunit 3-like protein (Ycg1)
Mol. type   Protein
Organism   Chaetomium thermophilum
Olig. state   Monomer
Mon. MW   108.3 kDa
 
UniProt   G0SHK2 (24-1006)
Sequence   FASTA
 
PDB ID   5OQQ