Structural consequences of transforming growth factor beta-1 activation from near-therapeutic X-ray doses.

Stachowski T, Grant TD, Snell EH, J Synchrotron Radiat 26(Pt 4):967-979 (2019) Europe PMC

SASDFD2 – wild-type human Latent Transforming Growth Factor beta 1 (LTGFB-1)

Human Latent Transforming Growth Factor beta 1
MWexperimental 104 kDa
MWexpected 86 kDa
VPorod 200 nm3
log I(s) 6.69×100 6.69×10-1 6.69×10-2 6.69×10-3
Human Latent Transforming Growth Factor beta 1 small angle scattering data  s, nm-1
ln I(s)
Human Latent Transforming Growth Factor beta 1 Guinier plot ln 6.69×100 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
Human Latent Transforming Growth Factor beta 1 Kratky plot 1.104 0 3 sRg
p(r)
Human Latent Transforming Growth Factor beta 1 pair distance distribution function Rg: 4.1 nm 0 Dmax: 17.5 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of wild-type human Latent Transforming Growth Factor beta 1 (LTGFB-1) in phosphate buffered saline, 2% glycerol, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. 49 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Human Latent Transforming Growth Factor beta 1 (LTGFB-1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   43.0 kDa
 
UniProt   P01137 (30-390)
Sequence   FASTA