| MWI(0) | 122 | kDa |
| MWexpected | 125 | kDa |
| VPorod | 230 | nm3 |
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log I(s)
9.64×103
9.64×102
9.64×101
9.64×100
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s, nm-1
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Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1.
Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI, J Biol Chem 294(37):13822-13829 (2019) Europe PMCSASDFD4 – Condensin complex subunit 3-like protein,Ycg1, bound to condensin complex subunit 2, Brn1 (Ycg1-Brn1)
Condensin complex subunit 3-like proteinCondensin complex subunit 2
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Fits and models
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Synchrotron SAXS data from solutions of the Ycg1-Brn1 complex in 25 mM Tris 300 mM NaCl 1mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 5 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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