| MWexperimental | 109 | kDa |
| MWexpected | 105 | kDa |
| VPorod | 147 | nm3 |
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log I(s)
6.11×102
6.11×101
6.11×100
6.11×10-1
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s, nm-1
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Studying Conformational Changes of the Yersinia Type-III-Secretion Effector YopO in Solution by Integrative Structural Biology.
Peter MF, Tuukkanen AT, Heubach CA, Selsam A, Duthie FG, Svergun DI, Schiemann O, Hagelueken G, Structure 27(9):1416-1426.e3 (2019) Europe PMCSASDFD6 – Protein kinase YopO - cytoplasmic 1 actin complex
Protein kinase YopOActin, cytoplasmic 1
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Fits and models
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Synchrotron SAXS data from solutions of protein kinase YopO in complex with actin in 10 mM Tris-HCl, 50 mM NaCl, pH 8 were collected on the EMBL P12 beam line at the PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAXS was employed. The SEC parameters were as follows: A 80.00 μl sample at 5.5 mg/ml was injected onto a GE Superdex 200 Increase 10/300 column at 20°C. 3600 successive 1 second frames were collected through the SEC elution. The data from the sample peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Flow rate = UNKNOWN |
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