Structural consequences of transforming growth factor beta-1 activation from near-therapeutic X-ray doses.

Stachowski T, Grant TD, Snell EH, J Synchrotron Radiat 26(Pt 4):967-979 (2019) Europe PMC

SASDFE2 – wild-type human Latency Associated Peptide (LAP)

Latency Associated Peptide
MWexperimental 91 kDa
MWexpected 58 kDa
VPorod 179 nm3
log I(s) 2.81×101 2.81×100 2.81×10-1 2.81×10-2
Latency Associated Peptide small angle scattering data  s, nm-1
ln I(s)
Latency Associated Peptide Guinier plot ln 2.82×101 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Latency Associated Peptide Kratky plot 1.104 0 √3 sRg
p(r)
Latency Associated Peptide pair distance distribution function Rg: 4.4 nm 0 Dmax: 17.5 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of wild-type human Latency Associated Peptide (LAP) in phosphate buffered saline, pH 7.4 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.50 mg/ml was measured at 10°C. Four successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Latency Associated Peptide (LAP)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   29.2 kDa
 
UniProt   P01137 (30-278)
Sequence   FASTA