Synchrotron SAXS
data from solutions of
Pseudomonas putida CBB5 NdmA hexamer
in
20 mM HEPES 150 mM NaCl 2 mM TCEP, pH 7.5
were collected
on the
BL-10C beam line
at the Photon Factory (PF), High Energy Accelerator Research Organization (KEK) storage ring
(Tsukuba, Japan)
using a Pilatus3 2M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 200.00 μl sample
at 12.1 mg/ml was injected at a 0.05 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 18°C.
32 successive
10 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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