| MWexperimental | 444 | kDa |
| MWexpected | 415 | kDa |
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log I(s)
2.19×102
2.19×101
2.19×100
2.19×10-1
|
s, nm-1
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Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex.
Kim JH, Kim BH, Brooks S, Kang SY, Summers RM, Song HK, J Mol Biol 431(19):3647-3661 (2019) Europe PMCSASDFJ7 – Pseudomonas putida CBB5 mCherry-NdmA/ECFP-NdmB complex - static
Methylxanthine N1-demethylase NdmAMethylxanthine N3-demethylase NdmB
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Fits and models
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Synchrotron SAXS
data from solutions of
Pseudomonas putida CBB5 mCherry-NdmA/ECFP-NdmB complex - static
in
20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH 7.5
were collected
on the
4C beam line
at the Pohang Accelerator Laboratory storage ring
(Pohang, South Korea)
using a Rayonix SX165 detector
at a wavelength of λ = 0.0733 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 4.4 and 11 mg/ml were measured
at 4°C.
Six successive
5 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
Sample detector distance = UNKNOWN |
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