Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1.

Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI, J Biol Chem 294(37):13822-13829 (2019) Europe PMC

SASDFK4 – Condensin complex subunit 3-like protein, Ycg1, at 0.25mg/ml

Condensin complex subunit 3-like protein
Condensin complex subunit 3-like protein
MWI(0) 138 kDa
MWexpected 325 kDa
VPorod 280 nm3
log I(s) 1.07×104 1.07×103 1.07×102 1.07×101
Condensin complex subunit 3-like protein Condensin complex subunit 3-like protein small angle scattering data  s, nm-1
ln I(s)
Condensin complex subunit 3-like protein Condensin complex subunit 3-like protein Guinier plot ln 1.07×104 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Condensin complex subunit 3-like protein Condensin complex subunit 3-like protein Kratky plot 1.104 0 √3 sRg
p(r)
Condensin complex subunit 3-like protein Condensin complex subunit 3-like protein pair distance distribution function Rg: 4.6 nm 0 Dmax: 15.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Condensin complex subunit 3-like protein Condensin complex subunit 3-like protein SASREF MX model
Condensin complex subunit 3-like protein Condensin complex subunit 3-like protein SREFLEX model
Synchrotron SAXS data from solutions of Ycg1 in 25 mM Tris 300 mM NaCl 1mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.25 mg/ml was measured at 10.1°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Ycg1 at 0.25 mg/ml was modeled as a volume-fraction weighted mixture consisting of 66% monomers and 34% dimers

Condensin complex subunit 3-like protein (Ycg1)
Mol. type   Protein
Organism   Chaetomium thermophilum
Olig. state   Monomer
Mon. MW   108.3 kDa
 
UniProt   G0SHK2 (24-1006)
Sequence   FASTA
 
Condensin complex subunit 3-like protein (Ycg1)
Mol. type   Protein
Organism   Chaetomium thermophilum
Olig. state   Dimer
Mon. MW   108.3 kDa
 
UniProt   G0SHK2 (24-1006)
Sequence   FASTA
 
PDB ID   5OQQ
 
PDB ID   5OQQ