Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.

Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B, J Struct Biol :107429 (2019) Europe PMC

SASDFM2 – Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 275-279)

Cell wall synthesis protein Wag31
MWexperimental 30 kDa
MWexpected 30 kDa
log I(s) 1.90×101 1.90×100 1.90×10-1 1.90×10-2
Cell wall synthesis protein Wag31 small angle scattering data  s, nm-1
ln I(s)
Cell wall synthesis protein Wag31 Guinier plot ln 1.90×101 Rg: 9.8 nm 0 (9.8 nm)-2 s2
(sRg)2I(s)/I(0)
Cell wall synthesis protein Wag31 Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 275-279) in 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH 7.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.40 mg/ml was measured at 23°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak. SEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 275-279

Cell wall synthesis protein Wag31
Mol. type   Protein
Organism   Mycobacterium tuberculosis
Olig. state   Unknown
Mon. MW   30.0 kDa
 
UniProt   P9WMU1
Sequence   FASTA