Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.

Narayanan N, Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT, J Mol Biol (2019) Europe PMC

SASDFM6 – Adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5

Adenine specific DNA methyltransferase (Mod)

MW^experimental	250	kDa
MW^expected	273	kDa
V^Porod	316	nm³

log I(s) 1.26×10² 1.26×10¹ 1.26×10⁰ 1.26×10^-1

Adenine specific DNA methyltransferase (Mod) small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Adenine specific DNA methyltransferase (Mod) Guinier plot

ln 1.27×10² R_g: 5.0 nm 0 (5.0 nm)^-2 s²

(sR_g)²I(s)/I(0)

Adenine specific DNA methyltransferase (Mod) Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 4.9 nm 0 D_max: 19.1 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.7

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.255
0.894

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Adenine specific DNA methyltransferase (Mod) PYMOL model

Synchrotron SAXS data from solutions of adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5 in 25 mM citrate, 250 mM NaCl were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The sample is severely aggregated. Wavelength UNKNOWN; Experimental temperature, UNKNOWN; Exposure time, UNKNOWN; Sample to detector distance, UNKNOWN.

Adenine specific DNA methyltransferase (Mod)
Mol. type		Protein
Organism		Helicobacter pylori
Olig. state		Tetramer
Mon. MW		68.4 kDa

UniProt		O25315 (1-598)
Sequence		FASTA