SASDFR4 – HP2042 form from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, Trp repressor from Escherichia coli (Domain A (PDB:3MLI)-Linker (PDB:1BR0)-Domain B (PDB:1WRT))

MWI(0) 177 kDa MWexpected 150 kDa VPorod 379 nm3 log I(s) 3.72×101 3.72×100 3.72×10-1 3.72×10-2  s, nm-1 Log plot Log-Log plot

ln I(s) ln 3.72×101 Rg: 5.1 nm 0 (5.1 nm)-2 s2 (sRg)2I(s)/I(0) 1.104 0 √3 sRg p(r) Rg: 5.2 nm 0 Dmax: 20 nm

Fits and models

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log I(s)  s, nm-1 +3 Δ/σ -3  s, nm-1 Fit validation Metric Value pcormap χ² 0.046 0.100 Worse Better

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SAXS data from solutions of the fusion protein1 in 20 mM Tris-HCl, 150 mM KCl, pH 8.0 were collected using a Nano-Viewer (Rigaku, Japan) at NAIST (Nara, Japan) equipped with a Pilatus 200K detector at a sample-detector distance of 0.75 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Twenty-five successive 60 second frames were collected at a sample temperature of 20°C. Solute concentrations ranging between 2.3 and 8.3 mg/ml were measured. The data were normalized to the intensity of the direct beam and radially averaged. The scattering from the solvent-blank was subtracted. The scattering profile in this entry represents an extrapolation to infinite dilution.

HP2042 form from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, Trp repressor from Escherichia coli (Fusion protein1) Mol. type   Protein Organism   Helicobacter pylori, Rattus norvegicus, Escherichia coli Olig. state   Tetramer Mon. MW   37.4 kDa Sequence   FASTA   PDB ID   3MLI   PDB ID   1BR0   PDB ID   1WRT