SASDFS4 – HP0242 from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, de novo designed coiled-coil trimer domain (Domain A (PDB:3MLI)-Linker (PDB:1BR0)-Domain B (PDB:4DZL))

MWI(0) 187 kDa MWexpected 179 kDa VPorod 642 nm3 log I(s) 4.01×101 4.01×100 4.01×10-1 4.01×10-2  s, nm-1 Log plot Log-Log plot

ln I(s) ln 4.01×101 Rg: 6.5 nm 0 (6.5 nm)-2 s2 (sRg)2I(s)/I(0) 1.104 0 √3 sRg p(r) Rg: 7 nm 0 Dmax: 24 nm

Fits and models

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log I(s)  s, nm-1 +3 Δ/σ -3  s, nm-1 Fit validation Metric Value pcormap χ² 0.000 0.100 Worse Better

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SAXS data from solutions of the fusion protein2 in 20 mM Tris-HCl, 150 mM KCl, pH 8.0 were collected using a Nano-Viewer (Rigaku, Japan) at NAIST (Nara, Japan) equipped with a Pilatus 200K detector at a sample-detector distance of 0.75 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Twenty-five successive 60 second frames were collected at a sample temperature of 20°C. Solute concentrations ranging between 1.9 and 6.8 mg/ml were measured. The data were normalized to the intensity of the direct beam and radially averaged. The scattering from the solvent-blank was subtracted. The scattering profile in this entry represents an extrapolation to infinite dilution.

HP0242 from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, de novo designed coiled-coil trimer domain (Fusion protein2) Mol. type   Protein Organism   Helicobacter pylori, Rattus norvegicus, de novo design Olig. state   Hexamer Mon. MW   29.9 kDa Sequence   FASTA   PDB ID   3MLI   PDB ID   1BR0   PDB ID   4DZL