| MWexperimental | 290 | kDa |
| MWexpected | 316 | kDa |
| VPorod | 386 | nm3 |
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log I(s)
3.75×101
3.75×100
3.75×10-1
3.75×10-2
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s, nm-1
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Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M, Nat Commun 11(1):2905 (2020) Europe PMCSASDFS6 – DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:2.5)
80bp_DNA Forward80bp_DNA Reverse
DNA-binding protein HU-alpha, E34K
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Fits and models
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Synchrotron SAXS
data from solutions of
DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:2.5)
in
20mM HEPES, 100mM NaCl, pH 7.5
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
at 10°C.
300 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN. Concentration = UNKNOWN |
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