Synchrotron SAXS
data from solutions of
Adhesion G-protein coupled receptor G6 - zfGpr126 S2 (-ss) ECR
in
150 mM NaCl, 20 mM HEPES, pH 7.5
were collected
on the
BioCAT 18ID beam line
at the Advanced Photon Source (APS), Argonne National Laboratory storage ring
(Lemont, IL, USA)
using a Pilatus3 X 1M detector
at a sample-detector distance of 1 m and
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 200.00 μl sample
at 5 mg/ml was injected at a 0.70 ml/min flow rate
onto a GE Superdex 200 Increase 10/300 column
at 22°C.
1266 successive
0.800 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
