The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering.

Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orłowski A, Jänis J, Määttä JAE, Varjosalo M, Vattulainen I, Róg T, Svergun D, Cheng RH, Wu J, Hytönen VP, Wehrle-Haller B, J Cell Sci 133(19) (2020) Europe PMC

SASDFU7 – Talin-1 head amino acids 1-405(Δ134-170/GAG insert)

Talin-1 (Δ134-170/GAG insert), human
MWI(0) 59 kDa
MWexpected 47 kDa
VPorod 71 nm3
log I(s) 3.28×103 3.28×102 3.28×101 3.28×100
Talin-1 (Δ134-170/GAG insert), human small angle scattering data  s, nm-1
ln I(s)
Talin-1 (Δ134-170/GAG insert), human Guinier plot ln 3.29×103 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
Talin-1 (Δ134-170/GAG insert), human Kratky plot 1.104 0 √3 sRg
p(r)
Talin-1 (Δ134-170/GAG insert), human pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Talin-1 head amino acids 1-405(Δ134-170/GAG insert) Rg histogram Rg, nm
Talin-1 (Δ134-170/GAG insert), human EOM/RANCH model
Talin-1 (Δ134-170/GAG insert), human EOM/RANCH model
Talin-1 (Δ134-170/GAG insert), human EOM/RANCH model
Synchrotron SAXS data from solutions of Talin-1 head amino acids 1-405(Δ134-170/GAG insert) in 50 mM sodium phosphate, 150mM NaCl, pH 7.2 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.12399 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 8.2 mg/ml was injected at a 0.25 ml/min flow rate onto a column. 20 successive 0.995 second frames were collected through the sample peak (from a total of 2400) at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SEC column = UNKNOWN

Talin-1 (Δ134-170/GAG insert), human (TLN1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   47.0 kDa
 
UniProt   Q9Y490 (1-405)
Sequence   FASTA