Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)

Soumya G Remesh.

SASDFV6 – DNA-binding protein HU-alpha, E38K/V42L double mutant

DNA-binding protein HU-alpha, E38K/V42L double mutant
MWexperimental 60 kDa
MWexpected 95 kDa
VPorod 53 nm3
log I(s) 5.99×101 5.99×100 5.99×10-1 5.99×10-2
DNA-binding protein HU-alpha, E38K/V42L double mutant small angle scattering data  s, nm-1
ln I(s)
DNA-binding protein HU-alpha, E38K/V42L double mutant Guinier plot ln 6.00×101 Rg: 3.0 nm 0 (3.0 nm)-2 s2
(sRg)2I(s)/I(0)
DNA-binding protein HU-alpha, E38K/V42L double mutant Kratky plot 1.104 0 √3 sRg
p(r)
DNA-binding protein HU-alpha, E38K/V42L double mutant pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DNA-binding protein HU-alpha, E38K/V42L double mutant CHIMERA model
DNA-binding protein HU-alpha, E38K/V42L double mutant CHIMERA model
Synchrotron SAXS data from solutions of DNA-binding protein HU-alpha, E38K/V42L double mutant in 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a MAR 165 CCD detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). at 10°C. 32 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Concentration = UNKNOWN

DNA-binding protein HU-alpha, E38K/V42L double mutant
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Decamer
Mon. MW   9.5 kDa
 
UniProt   P0ACF0 (1-90)
Sequence   FASTA