| MWexperimental | 62 | kDa |
| MWexpected | 88 | kDa |
| VPorod | 297 | nm3 |
|
log I(s)
5.56×102
5.56×101
5.56×100
5.56×10-1
|
s, nm-1
|
Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh.SASDFW6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:2)
80bp_DNA Forward80bp_DNA Reverse
DNA-binding protein HU-alpha, E38K/V42L double mutant
|
|
|
Fits and models
|
|
|
Synchrotron SAXS
data from solutions of
DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:2)
in
50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH 7.5
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a MAR 165 CCD detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.1 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
at 10°C.
32 successive
0.100 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN. Concentration = UNKNOWN |
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||