Structural insights into rice SalTol QTL located SALT protein.

Kaur N, Sagar A, Sharma P, Ashish, Pati PK, Sci Rep 10(1):16589 (2020) Europe PMC

SASDG27 – Salt stress-induced protein at a sample concentration of 22mg/ml

Salt stress-induced protein
MWexperimental 42 kDa
MWexpected 37 kDa
VPorod 57 nm3
log I(s) 8.05×104 8.05×103 8.05×102 8.05×101
Salt stress-induced protein small angle scattering data  s, nm-1
ln I(s)
Salt stress-induced protein Guinier plot ln 8.06×104 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Salt stress-induced protein Kratky plot 1.104 0 √3 sRg
p(r)
Salt stress-induced protein pair distance distribution function Rg: 2.3 nm 0 Dmax: 6.5 nm

Data validation

There are no models related to this curve.

SAXS data from solutions of salt stress-induced protein in 50 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4 were collected using an Anton Paar SAXSpace instrument (CSIR - Institute of Microbial Technology (IMTech), Chandigarh, India) equipped with a Mythen 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 22.00 mg/ml was measured at 10°C. Three successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Salt stress-induced protein (SALT)
Mol. type   Protein
Organism   Oryza sativa Indica Group
Olig. state   Dimer
Mon. MW   18.7 kDa
 
UniProt   A2WPN7 (1-145)
Sequence   FASTA