The guide sRNA sequence determines the activity level of box C/D RNPs.

Graziadei A, Gabel F, Kirkpatrick J, Carlomagno T, Elife 9 (2020) Europe PMC

SASDG43 – The Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D and 2H Fibrillarin (SANS data in 42% v/v D2O)

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
50S ribosomal protein L7Ae
NOP5/NOP56 related protein
pyrococcus furiosus sR26 stabilized construct
Pyrococcus furiosus sR26 substrate D
MWexperimental 200 kDa
MWexpected 200 kDa
VPorod 65 nm3
log I(s) 4.60×10-1 4.60×10-2 4.60×10-3 4.60×10-4
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D small angle scattering data  s, nm-1
ln I(s)
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Guinier plot ln 4.60×10-1 Rg: 4.9 nm 0 (4.9 nm)-2 s2
(sRg)2I(s)/I(0)
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D Kratky plot 1.104 0 3 sRg
p(r)
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase 50S ribosomal protein L7Ae NOP5/NOP56 related protein pyrococcus furiosus sR26 stabilized construct Pyrococcus furiosus sR26 substrate D pair distance distribution function Rg: 5.4 nm 0 Dmax: 16.6 nm

Data validation


There are no models related to this curve.

SANS data from solutions of the Pyrococcus furiosus Box C/D sRNP complex reconstituted with 1H Nop5, 1H L7Ae, 1H st-sR26 RNA, 1H substrate D and 2H Fibrillarin in 50 mM phosphate 500 mM NaCl 42%D2O, pH 6.6 were collected on KWS1 (FRM2; Munich, Germany) using a 6Li-Scintillator 1 mm thickness + photomultiplier detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.5 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 7.70 mg/ml was measured at 55°C. One 7200 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase (Fibrillarin)
Mol. type   Protein
Organism   Pyrococcus furiosus
Olig. state   Dimer
Mon. MW   25.8 kDa
 
UniProt   Q8U4M2 (1-227)
Sequence   FASTA
 
50S ribosomal protein L7Ae (L7Ae)
Mol. type   Protein
Organism   Pyrococcus furiosus
Olig. state   Dimer
Mon. MW   13.4 kDa
 
UniProt   Q8U160 (1-123)
Sequence   FASTA
 
NOP5/NOP56 related protein (Nop5)
Mol. type   Protein
Organism   Pyrococcus furiosus
Olig. state   Dimer
Mon. MW   46.8 kDa
 
UniProt   Q8U4M1 (1-402)
Sequence   FASTA
 
pyrococcus furiosus sR26 stabilized construct (st-sR26)
Mol. type   RNA
Organism   Pyrococcus furiosus
Olig. state   Monomer
Mon. MW   23.5 kDa
Sequence   FASTA
 
Pyrococcus furiosus sR26 substrate D (Substrate D)
Mol. type   RNA
Organism   Pyrococcus furiosus
Olig. state   Monomer
Mon. MW   4.5 kDa
Sequence   FASTA