| MWexperimental | 119 | kDa |
| MWexpected | 124 | kDa |
| VPorod | 188 | nm3 |
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log I(s)
2.55×102
2.55×101
2.55×100
2.55×10-1
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s, nm-1
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Intermediate-resolution crystal structure of the human adenovirus B serotype 3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2.
Vassal-Stermann E, Hutin S, Fender P, Burmeister WP, Acta Crystallogr F Struct Biol Commun 75(Pt 12):750-757 (2019) Europe PMCSASDG44 – Adenovirus Ad3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2
Human adenovirus serotype 3 fibre knobHuman desmoglein 2 extracellular domain 2 and 3
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Fits and models
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Synchrotron SAXS data from solutions of adenovirus Ad3 fibre knob in complex with the EC2-EC3 fragment of desmoglein 2 in 20 mM Tris-HCl, 150 mM NaCl, 3 mM CaCl2, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 2.4 mg/ml was injected at a 0.10 ml/min flow rate onto a GE Superdex 200 Increase 3.2/300 column at 293°C. 2000 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Three different stoichiometries are possible for the Had3K: human desmoglein 2 EC2-EC3 complex : 3:1, 3:2 and 3:3. The SAXS experiment is used in order to determine the possible stoichiometries in solution. |
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