Mannose receptor (CD206) activation in tumor-associated macrophages enhances adaptive and innate antitumor immune responses.

Jaynes JM, Sable R, Ronzetti M, Bautista W, Knotts Z, Abisoye-Ogunniyan A, Li D, Calvo R, Dashnyam M, Singh A, Guerin T, White J, Ravichandran S, Kumar P, Talsania K, Chen V, Ghebremedhin A, Karanam B, Bin Salam A, Amin R, Odzorig T, Aiken T, Nguyen V, Bian Y, Zarif JC, de Groot AE, Mehta M, Fan L, Hu X, Simeonov A, Pate N, Abu-Asab M, Ferrer M, Southall N, Ock CY, Zhao Y, Lopez H, Kozlov S, de Val N, Yates CC, Baljinnyam B, Marugan J, Rudloff U, Sci Transl Med 12(530) (2020) Europe PMC

SASDG54 – Human macrophage mannose receptor 1 protein

Macrophage mannose receptor 1

MW^experimental	345	kDa
MW^expected	315	kDa
V^Porod	584	nm³

log I(s) 5.30×10^-1 5.30×10^-2 5.30×10^-3 5.30×10^-4

Macrophage mannose receptor 1 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Macrophage mannose receptor 1 Guinier plot

ln 5.30×10^-1 R_g: 7.9 nm 0 (7.9 nm)^-2 s²

(sR_g)²I(s)/I(0)

Macrophage mannose receptor 1 Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 8.2 nm 0 D_max: 30.1 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.5

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.592
0.049

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Macrophage mannose receptor 1 ITASSER model

Synchrotron SAXS data from solutions of human macrophage mannose receptor 1 in 50 mM HEPES, 100 mM NaCl, 1 mM DTT, pH 7 were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.093 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.9 and 1.8 mg/ml were measured at 20°C. 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Macrophage mannose receptor 1 (MRC1 or CD206)
Mol. type		Protein
Organism		Mouse myeloma cell line
Olig. state		Dimer
Mon. MW		157.5 kDa

UniProt		P22897 (19-1388)
Sequence		FASTA