A highly stable, non-digestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs.

Brola TR, Dreon MS, Qiu JW, Heras H, J Exp Biol 223(Pt 19) (2020) Europe PMC

SASDG75 – Pomacea diffusa perivitellin-1

Pomacea diffusa perivitellin-1
MWexperimental 465 kDa
MWexpected 23 kDa
VPorod 529 nm3
log I(s) 8.56×10-4 8.56×10-5 8.56×10-6 8.56×10-7
Pomacea diffusa perivitellin-1 small angle scattering data  s, nm-1
ln I(s)
Pomacea diffusa perivitellin-1 Guinier plot ln 8.56×10-4 Rg: 4.6 nm 0 (4.6 nm)-2 s2
(sRg)2I(s)/I(0)
Pomacea diffusa perivitellin-1 Kratky plot 1.104 0 √3 sRg
p(r)
Pomacea diffusa perivitellin-1 pair distance distribution function Rg: 4.7 nm 0 Dmax: 15.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Pomacea diffusa perivitellin-1 DAMMIF model
log I(s)
 s, nm-1
Pomacea diffusa perivitellin-1 DAMMIF model
log I(s)
 s, nm-1
Pomacea diffusa perivitellin-1 DAMMIF model
Synchrotron SAXS data from solutions of Pomacea diffusa perivitellin-1 in 50 mM phosphate buffer, pH 7.4 were collected on the SAXS2 beam line at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a MAR 165 CCD detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.155 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.65 mg/ml was measured at 25°C. Five successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Pomacea diffusa perivitellin-1 (PdPV1)
Mol. type   Protein
Organism   Pomacea diffusa
Olig. state   Unknown
Mon. MW   22.8 kDa
Sequence   FASTA