Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis.

Zhou Y, Millott R, Kim HJ, Peng S, Edwards RA, Skene-Arnold T, Hammel M, Lees-Miller SP, Tainer JA, Holmes CFB, Glover JNM, Structure 27(10):1485-1496.e4 (2019) Europe PMC

SASDG96 – Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Apoptosis-stimulating of p53 protein 2
MWexperimental 57 kDa
MWexpected 64 kDa
VPorod 144 nm3
log I(s) 1.77×103 1.77×102 1.77×101 1.77×100
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Apoptosis-stimulating of p53 protein 2 small angle scattering data  s, nm-1
ln I(s)
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Apoptosis-stimulating of p53 protein 2 Guinier plot ln 1.78×103 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Apoptosis-stimulating of p53 protein 2 Kratky plot 1.104 0 3 sRg
p(r)
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Apoptosis-stimulating of p53 protein 2 pair distance distribution function Rg: 3.0 nm 0 Dmax: 10.2 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Apoptosis-stimulating of p53 protein 2 BILBOMD model
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Apoptosis-stimulating of p53 protein 2 BILBOMD model
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Apoptosis-stimulating of p53 protein 2 BILBOMD model

Synchrotron SAXS data from solutions of Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact in 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample was injected at a 0.50 ml/min flow rate onto a Shodex KW-800 series column at 20°C. 800 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample injection volume = UNKNOWN

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PP-1cα)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   37.5 kDa
 
UniProt   P62136 (1-330)
Sequence   FASTA
 
Apoptosis-stimulating of p53 protein 2 (ASPP2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   26.1 kDa
 
UniProt   Q13625 (905-1128)
Sequence   FASTA
 
PDB ID   6DCX
 
PDB ID   6DCX
 
PDB ID   6DCX