Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.

Sajko S, Grishkovskaya I, Kostan J, Graewert M, Setiawan K, Trübestein L, Niedermüller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K, PLoS One 15(12):e0242677 (2020) Europe PMC

SASDG97 – Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 2-15

Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15
MWexperimental 74 kDa
MWexpected 78 kDa
VPorod 187 nm3
log I(s) 5.42×101 5.42×100 5.42×10-1 5.42×10-2
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 small angle scattering data  s, nm-1
ln I(s)
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 Guinier plot ln 5.42×101 Rg: 6.5 nm 0 (6.5 nm)-2 s2
(sRg)2I(s)/I(0)
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 Kratky plot 1.104 0 √3 sRg
p(r)
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 pair distance distribution function Rg: 7.3 nm 0 Dmax: 26 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 DAMMIF model
log I(s)
 s, nm-1
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 CUSTOM IN-HOUSE model
Synchrotron SAXS data from solutions of Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 2-15 in 20 mM Tris-HCl, 100 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample at 3.8 mg/ml was injected at a 0.50 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 90 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 (tbMORNdelta1)
Mol. type   Protein
Organism   Trypanosoma brucei
Olig. state   Dimer
Mon. MW   39.2 kDa
 
UniProt   Q587D3 (26-358)
Sequence   FASTA