MWexperimental | 26 | kDa |
MWexpected | 24 | kDa |
VPorod | 49 | nm3 |
log I(s)
1.76×101
1.76×100
1.76×10-1
1.76×10-2
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s, nm-1
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Synchrotron SAXS data from solutions of the C-terminal cell-surface signaling domain of PupR in 25 mM HEPES 400 mM LiCl 10% v/v glycerol, pH 7.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 500.00 μl sample at 9.6 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of an appropriate solvent-blank was subtracted from the SEC elution peak of the sample.
Storage temperature = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN |
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