| MWexperimental | 84 | kDa |
| MWexpected | 80 | kDa |
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log I(s)
2.60×10-3
2.60×10-4
2.60×10-5
2.60×10-6
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s, nm-1
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Large-scale conformational rearrangement of the α5-helix of Gα subunits in complex with the guanine nucleotide exchange factor Ric8A.
Srivastava D, Artemyev NO, J Biol Chem (2019) Europe PMCSASDGB6 – Resistance to inhibitors of cholinesterase 8 homolog A (Ric8A) miniGi complex
Resistance to inhibitors of cholinesterase 8 homolog AminiGi
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Fits and models
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Synchrotron SAXS data from solutions of the Ric8A-miniGi complex in 20 mM Tris, 150 mM KCl, 5 % glycerol, 1 mM TCEP, pH 8 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 250.00 μl sample at 10 mg/ml was injected at a 0.90 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column . The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Purified Ric8a1-492 was mixed with excess of purified miniGi and the complex was purified by size-exclusion chromatography (SEC). The complex fractions were pooled together, concentrated and additional SEC-SAXS data was collected as described above. X-ray wavelength = UNKNOWN. |
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