Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery

Nasta V, Da Vela S, Gourdoupis S, Ciofi-Baffoni S, Svergun D, Banci L, Scientific Reports 9(1) (2019) DOI

SASDGE2 – Human mitochondrial iron-sulfur cluster assembly ISCA2 protein

Iron-sulfur cluster assembly 2 homolog, mitochondrial
MWexperimental 19 kDa
MWexpected 24 kDa
VPorod 31 nm3
log I(s) 8.90×101 8.90×100 8.90×10-1 8.90×10-2
Iron-sulfur cluster assembly 2 homolog, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Iron-sulfur cluster assembly 2 homolog, mitochondrial Guinier plot ln 8.90×101 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Iron-sulfur cluster assembly 2 homolog, mitochondrial Kratky plot 1.104 0 √3 sRg
p(r)
Iron-sulfur cluster assembly 2 homolog, mitochondrial pair distance distribution function Rg: 2.1 nm 0 Dmax: 6.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Iron-sulfur cluster assembly 2 homolog, mitochondrial SASREF model
log I(s)
 s, nm-1
Iron-sulfur cluster assembly 2 homolog, mitochondrial MODELLER model
log I(s)
 s, nm-1
Iron-sulfur cluster assembly 2 homolog, mitochondrial MODELLER model
log I(s)
 s, nm-1
Iron-sulfur cluster assembly 2 homolog, mitochondrial MOLMOL model
log I(s)
 s, nm-1
Iron-sulfur cluster assembly 2 homolog, mitochondrial MONSA model
Synchrotron SAXS data from solutions of human ISCA2 protein in 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample was injected at a 0.75 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 2400 successive 0.995 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Iron-sulfur cluster assembly 2 homolog, mitochondrial (ISCA2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   12.0 kDa
 
UniProt   Q86U28 (44-154)
Sequence   FASTA