Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery

Nasta V, Da Vela S, Gourdoupis S, Ciofi-Baffoni S, Svergun D, Banci L, Scientific Reports 9(1) (2019) DOI

SASDGF2 – Putative mitochondrial transferase CAF17 bound to mitochondrial iron-sulfur cluster assembly ISCA2 protein (human IBA57-ISCA2 complex)

Putative transferase CAF17, mitochondrial
Iron-sulfur cluster assembly 2 homolog, mitochondrial
MWI(0) 80 kDa
MWexpected 57 kDa
VPorod 114 nm3
log I(s) 6.19×10-2 6.19×10-3 6.19×10-4 6.19×10-5
Putative transferase CAF17, mitochondrial Iron-sulfur cluster assembly 2 homolog, mitochondrial small angle scattering data  s, nm-1
ln I(s)
Putative transferase CAF17, mitochondrial Iron-sulfur cluster assembly 2 homolog, mitochondrial Guinier plot ln 6.20×10-2 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Putative transferase CAF17, mitochondrial Iron-sulfur cluster assembly 2 homolog, mitochondrial Kratky plot 1.104 0 √3 sRg
p(r)
Putative transferase CAF17, mitochondrial Iron-sulfur cluster assembly 2 homolog, mitochondrial pair distance distribution function Rg: 4.3 nm 0 Dmax: 16.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Putative transferase CAF17, mitochondrial Iron-sulfur cluster assembly 2 homolog, mitochondrial SASREF CV model
log I(s)
 s, nm-1
Putative transferase CAF17, mitochondrial Iron-sulfur cluster assembly 2 homolog, mitochondrial MONSA model
Synchrotron SAXS data from solutions of the human IBA57-ISCA2 protein complex in 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.90 mg/ml was measured at 20°C. 40 successive 0.095 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Putative transferase CAF17, mitochondrial (IBA57)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   33.2 kDa
 
UniProt   Q5T440 (44-352)
Sequence   FASTA
 
Iron-sulfur cluster assembly 2 homolog, mitochondrial (ISCA2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   12.0 kDa
 
UniProt   Q86U28 (44-154)
Sequence   FASTA