G-quadruplex from HBV genome

Trushar Patel.

SASDGF6 – G-quadruplex DNA from Hepatitis B virus (mutant)

G-quadruplex mutant
MWexperimental 6 kDa
MWexpected 6 kDa
VPorod 11 nm3
log I(s) 3.30×10-3 3.30×10-4 3.30×10-5 3.30×10-6
G-quadruplex mutant small angle scattering data  s, nm-1
ln I(s)
G-quadruplex mutant Guinier plot ln 3.30×10-3 Rg: 1.7 nm 0 (1.7 nm)-2 s2
(sRg)2I(s)/I(0)
G-quadruplex mutant Kratky plot 1.104 0 √3 sRg
p(r)
G-quadruplex mutant pair distance distribution function Rg: 1.7 nm 0 Dmax: 5.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
G-quadruplex mutant DAMFILT model
Synchrotron SAXS data from solutions of G-quadruplex DNA from Hepatitis B virus (mutant) in 20 mM HEPES, 100 mM KCl, 1 mM EDTA, pH 7.5 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured. 620 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Cell temperature = UNKNOWN. Storage temperature = UNKNOWN

G-quadruplex mutant (mut)
Mol. type   DNA
Organism   Hepatitis B virus
Olig. state   Monomer
Mon. MW   5.8 kDa
Sequence   FASTA