Therapeutic approaches to ApoE

Lucas Kraft, University of Sussex PhD thesis 2019 (2019) URL

SASDGG9 – Apolipoprotein E4 bound to 1 mg/mL heparin (SEC-SAXS)

Apolipoprotein E4
Heparin
MWexperimental 230 kDa
MWexpected 154 kDa
log I(s) 2.70×10-1 2.70×10-2 2.70×10-3 2.70×10-4
Apolipoprotein E4 Heparin small angle scattering data  s, nm-1
ln I(s)
Apolipoprotein E4 Heparin Guinier plot ln 2.70×10-1 Rg: 6.2 nm 0 (6.2 nm)-2 s2
(sRg)2I(s)/I(0)
Apolipoprotein E4 Heparin Kratky plot 1.104 0 √3 sRg
p(r)
Apolipoprotein E4 Heparin pair distance distribution function Rg: 6.4 nm 0 Dmax: 21.6 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Apolipoprotein E4 bound to 1 mg/mL heparin (SEC-SAXS) in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 10 mg/ml was injected at a 0.16 ml/min flow rate onto a Shodex KW403-4F column at 20°C. Seven successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

ApoE4 at 10 mg/mL was gel-filtered in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, 1 mg/mL heparin, pH 8.0.

Apolipoprotein E4 (ApoE4)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Tetramer
Mon. MW   34.7 kDa
 
UniProt   P02649 (19-317)
Sequence   FASTA
 
Heparin
Mol. type   Other
Olig. state   Monomer
Mon. MW   15 kDa
Chemical formula