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SAXS data, I(s) vs s (s = 4π sin θ/λ, where 2θ is the scattering angle and λ is the X-ray wavelength) were collected from samples of mutated (F670E) bifunctional alcohol/aldehyde dehydrogenase protein (P0A9Q8) using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) on the B21 beamline at the Diamond Light Source (Oxfordshire, UK). Data (621 successive 3-second frames) were collected using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm. The SEC mobile phase consisted 50 mM HEPES pH 7, 500 mM NaCl, 5% (v/v) glycerol at 0.16 ml/min (4°C) with a sample injection concentration of 10 mg/ml. Data obtained from solute-free eluates and SEC-elution peak were normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted from the SEC-peak frames displaying constant estimated Rg values using ScÅtter and then analysed using Primus to produce the data displayed in this entry.
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s, nm-1
