Synchrotron SAXS
data from solutions of
Mutant Apolipoprotein E4 (K143A K146A) bound to 4 µg/mL heparin
in
20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Pilatus 2M detector
at a sample-detector distance of 4.0 m and
at a wavelength of λ = 0.1 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 4.00 mg/ml was measured
at 20°C.
28 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
ApoE4 (K143A K146A) mutant + 4 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.
s, nm-1
