Josts I,
Gao Y,
Monteiro DCF,
Niebling S,
Nitsche J,
Veith K,
Gräwert TW,
Blanchet CE,
Schroer MA,
Huse N,
Pearson AR,
Svergun DI,
Tidow H,
Structure
(2019)
Europe PMC
SASDGV5 – The nucleotide binding domain of Lipid A export ATP-binding/permease protein MsbA - data from stop-and-flow time-resolved SAXS (12 s time course)
Stop-and-flow time-resolved (TR) synchrotron SAXS data from solutions of MsbA in 20 mM Tris, 150 mM NaCl, 5 mM MgCl2 triggered by the introduction of 0.45 mM Mg2+-ATP, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). TR-SAXS were collected for a total of 400 successive 0.030 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The SAXS curve displayed in this entry corresponds to the last time point (12 s) of a stop-and-flow SAXS experiment monitoring the disassociation of NBD dimers into monomers caused by the introduction and subsequent hydrolysis of ATP. At 12 seconds through the time course the NBD becomes predominantly monomeric, with trace amounts of dimer. Attached to this entry, and made available in the full-entry zip archive, are all of the time-resolved stop-and-flow SAXS data out to the final 12 s time point.
s, nm-1
