| MWexperimental | 303 | kDa |
| MWexpected | 319 | kDa |
| VPorod | 457 | nm3 |
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log I(s)
2.69×101
2.69×100
2.69×10-1
2.69×10-2
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s, nm-1
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Combination of SAXS and Protein Painting Discloses the Three-Dimensional Organization of the Bacterial Cysteine Synthase Complex, a Potential Target for Enhancers of Antibiotic Action.
Rosa B, Marchetti M, Paredi G, Amenitsch H, Franko N, Benoni R, Giabbai B, De Marino MG, Mozzarelli A, Ronda L, Storici P, Campanini B, Bettati S, Int J Mol Sci 20(20) (2019) Europe PMCSASDGX6 – Cysteine synthase/Serine acetyltransferase complex
Cysteine synthase A (4-mer)Serine acetyltransferase (6-mer)
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Fits and models
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Synchrotron SAXS data from solutions of the cysteine synthase complex in 20 mM sodium phosphate, 85 mM NaCl, 2 mM EDTA, 10 mM 2-MCE, pH 7.5 were collected on the Austrian SAXS beamline 5.2L beam line at the ELETTRA - Sincrotrone Trieste storage ring (Trieste, Italy) using a Dectris / Pilatus3 1M detector at a sample-detector distance of 1.2 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 1.5 mg/ml were measured at 20°C. 12 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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